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psp64 poly vector  (Promega)

 
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    Structured Review

    Promega psp64 poly vector
    Successful insertion of rat HEV into <t>pSP64</t> poly (A) vector. A) Schematic representation of full-length genomic rat HEV insertion in the pSP64 poly (A) vector. A cDNA clone of rat HEV LCK-3110 strain was constructed and cloned into the pSP64 poly (A) vector using unique restriction sites, SaII and SacI. It contains EcoRI restriction sites at the 3′ end of the viral genome. B) Results from 1% gel electrophoresis demonstrating the insertion of the full-length rat HEV.
    Psp64 Poly Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psp64%28a%29+vector/pmc11259135-173-1-6?v=Promega
    Average 90 stars, based on 1 article reviews
    psp64 poly vector - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Rat hepatitis E virus cross-species infection and transmission in pigs"

    Article Title: Rat hepatitis E virus cross-species infection and transmission in pigs

    Journal: PNAS Nexus

    doi: 10.1093/pnasnexus/pgae259

    Successful insertion of rat HEV into pSP64 poly (A) vector. A) Schematic representation of full-length genomic rat HEV insertion in the pSP64 poly (A) vector. A cDNA clone of rat HEV LCK-3110 strain was constructed and cloned into the pSP64 poly (A) vector using unique restriction sites, SaII and SacI. It contains EcoRI restriction sites at the 3′ end of the viral genome. B) Results from 1% gel electrophoresis demonstrating the insertion of the full-length rat HEV.
    Figure Legend Snippet: Successful insertion of rat HEV into pSP64 poly (A) vector. A) Schematic representation of full-length genomic rat HEV insertion in the pSP64 poly (A) vector. A cDNA clone of rat HEV LCK-3110 strain was constructed and cloned into the pSP64 poly (A) vector using unique restriction sites, SaII and SacI. It contains EcoRI restriction sites at the 3′ end of the viral genome. B) Results from 1% gel electrophoresis demonstrating the insertion of the full-length rat HEV.

    Techniques Used: Plasmid Preparation, Construct, Clone Assay, Nucleic Acid Electrophoresis



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    Successful insertion of rat HEV into <t>pSP64</t> poly (A) vector. A) Schematic representation of full-length genomic rat HEV insertion in the pSP64 poly (A) vector. A cDNA clone of rat HEV LCK-3110 strain was constructed and cloned into the pSP64 poly (A) vector using unique restriction sites, SaII and SacI. It contains EcoRI restriction sites at the 3′ end of the viral genome. B) Results from 1% gel electrophoresis demonstrating the insertion of the full-length rat HEV.
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    Image Search Results


    Successful insertion of rat HEV into pSP64 poly (A) vector. A) Schematic representation of full-length genomic rat HEV insertion in the pSP64 poly (A) vector. A cDNA clone of rat HEV LCK-3110 strain was constructed and cloned into the pSP64 poly (A) vector using unique restriction sites, SaII and SacI. It contains EcoRI restriction sites at the 3′ end of the viral genome. B) Results from 1% gel electrophoresis demonstrating the insertion of the full-length rat HEV.

    Journal: PNAS Nexus

    Article Title: Rat hepatitis E virus cross-species infection and transmission in pigs

    doi: 10.1093/pnasnexus/pgae259

    Figure Lengend Snippet: Successful insertion of rat HEV into pSP64 poly (A) vector. A) Schematic representation of full-length genomic rat HEV insertion in the pSP64 poly (A) vector. A cDNA clone of rat HEV LCK-3110 strain was constructed and cloned into the pSP64 poly (A) vector using unique restriction sites, SaII and SacI. It contains EcoRI restriction sites at the 3′ end of the viral genome. B) Results from 1% gel electrophoresis demonstrating the insertion of the full-length rat HEV.

    Article Snippet: The plasmid pSP64 poly (A) vector (Promega) was used to clone the full-length rat HEV genome between the SalI and SacI sites (Fig. A).

    Techniques: Plasmid Preparation, Construct, Clone Assay, Nucleic Acid Electrophoresis

    Journal: iScience

    Article Title: Dry molten globule conformational state of CYP11A1 (SCC) regulates the first step of steroidogenesis in the mitochondrial matrix

    doi: 10.1016/j.isci.2024.110039

    Figure Lengend Snippet:

    Article Snippet: pSP64 -Vector , Promega , P1241.

    Techniques: Protease Inhibitor, RIA Assay, Plasmid Preparation, Expressing, Isolation, Lysis

    Key resources table

    Journal: iScience

    Article Title: Tom40 in cholesterol transport

    doi: 10.1016/j.isci.2023.106386

    Figure Lengend Snippet: Key resources table

    Article Snippet: StAR, SCC or the different deletional mutant cDNA plasmids were cloned in pSP64 vector (Promega), translated with 35 S-methionine or with cold methionine in TNT-rabbit reticulocyte system (Promega/Thermo Fisher) with SP6 polymerase at 30°C for 2 h. 47 Ribosomes and associated incompletely synthesized polypeptide chains were removed by ultracentrifugation at 148,000 ×g for 20 min at 4°C.

    Techniques: Protease Inhibitor, RIA Assay, Viability Assay, Recombinant, Plasmid Preparation, Expressing, Clear Native PAGE, Isolation